FAQs

Absorbance 96

Wavelength/Filters

Is the Absorbance 96 capable of a readout in the UV range?

No. The Absorbance 96 is a filter-based reader offering 4 discrete wavelengths in the range of 400 nm to 1000 nm.

Can I exchange the filters?

You cannot exchange the filters yourself.  However, the possibility of a filter exchange can be booked as part of our Care Packages. 

Can I order extra filters?

No, you cannot. The Absorbance 96 comes with four fixed filters. 

Is it possible to customize the wavelength combination?

Yes. You are free to choose a wavelength combination in the range of 400–1000 nm which best suits your assays. 

Is there a charge for customized wavelength combinations?

No, the customization itself comes at no extra cost. It may, however, increase the delivery time. If you need detailed information, just contact us.

Proper Use

Is an installation or setup by a technician necessary?

No. You must simply unbox the device, install the software, and the reader is ready to go within a few minutes.

Does the Absorbance 96 need to be maintained or serviced by a technician?

No, the Absorbance 96 is maintenance free.

How is the microplate inserted into the reader? Is there a tray?

You can insert the plate manually, i.e., by hand, into the reader. There is no sliding/automatic tray system.

Can a readout be performed with a lid on the micro plate?

No.

Which plate types can be used in the Absorbance 96?

The Absorbance 96 is designed to read 96-well microplates. All plates adhering to the ANSI standards should work with the device.

Can I use U-bottom or V-bottom plates?

Best results are obtained using flat-bottom plates. Our internal tests with U-bottom plates show the same performance with clear samples as compared to flat-bottom plates and can be recommend. If you plan to use V-bottom plates, you need to validate the performance of the reader-plate combination prior usage.

Is the Absorbance 96 robust enough for transport?

Yes. One of the main advantages of the Absorbance 96 is its robustness. In combination with its small size, these features make the Absorbance 96 highly portable.

How do I perform a calibration of the Absorbance 96?

The Absorbance 96 has many self-checks built in and does not need to be regularly checked for accuracy. However, we are aware that certain laboratory certifications require measurement devices to be regularly calibrated. For this purpose, we recommend the calibration plates from Hellma or our Care Packages, which offer a manufacturer calibration and corresponding certificate. 

Is IQ/OQ/PQ Documentation available for the Absorbance 96?

Yes, there is IQ/OQ/PQ Documentation available. Please contact us.

Can the Absorbance 96 be disinfected for sterile usage?

Yes. The Absorbance 96 can be disinfected using a cloth moistened with 70 % Ethanol solution.

Features/Performance

Can I measure fluorescence or luminescence with the Absorbance 96?

No. The Absorbance 96 is designed for absorbance (OD) measurements and is not suited to read luminescence or fluorescence. But let’s see what the future brings – if you want to stay in contact and be one of the first to hear about upcoming products and innovations, subscribe to our newsletter.

Does the Absorbance 96 offer plate-shaking?

No. However, Byonoy offers an adapter that can be used to place the Absorbance 96 on certain shakers. For more information contact us.

Does the Absorbance 96 offer temperature control?

No.

Can I place the Absorbance 96 in an incubator?

Yes, you can place the Absorbance 96 into an incubator. Since only one USB cable is used for power and data transfer, most incubators are suited for this purpose. Please ensure you adhere to the specifications for humidity and temperature.

Can I use the Absorbance 96 in an anaerobic chamber or glove box?

Yes, this is possible and encouraged! Thanks to its small size, the Absorbance 96 fits into space-restricted enclosures easily and offers multiple wavelengths to enable a range of assays to be performed. Many of our customers use the device in this fashion.

Is the open slot design susceptible to dust?

Under laboratory condition, only minimal dust exposure is expected to enter the slot, which will be factored out during the initialization procedure. Beyond that, the Absorbance 96 has an internal dust detection, which will warn you if too much dust is detected, thereby preventing any false results. If needed, you have the option to open the reader for a cleaning procedure.

Can ambient light disturb the read out?

Byonoy’s patent-pending ambient light canceling technology and advanced data processing enable the cancelation of the ambient light during the read out.

Is the Absorbance 96 verified/validated? Are there studies or comparisons with other readers?

The Absorbance 96 is being used by laboratory professionals across the world. Comparison data can be provided. If needed, just contact us.

Does the Absorbance 96 scan the microplate, i.e. have moving parts?

No, the Absorbance 96 is a robust, solid-state microplate reader, meaning it has no scanning mechanism. All wells are therefore read simultaneously without the need to move the plate during the readout. This technology offers you a very fast readout in under 5 seconds and prevents spillage.

Absorbance 96 Automate

Wavelength/Filters

Is there a charge for customized wavelength combinations?

No, the customization comes at no extra cost. It may, however, increase the delivery time.

Is Absorbance 96 Automate capable of a readout in the UV range?

No. The Absorbance 96 Automate Automate is a filter-based reader offering up to 6 discrete wavelengths in the range of 400 nm to 1000 nm. 

Can I exchange the filters?

Yes! Absorbance 96 Automate has exchangeable filter modules. To order a new wavelength, please contact us.  

Can I order extra filters?

Yes, you can. Absorbance 96 Automate comes with four standard filters and can accommodate up to 6. If you need to order a filter, contact us.

Is it possible to customize the wavelength combination?

Yes. You are free to choose a wavelength combination in the range of 400–1000 nm which best suits your assays. 

Proper Use

Can a readout be performed with a lid on the micro plate?

Yes.

Which plate types can be used in Absorbance 96 Automate?

Absorbance 96 Automate is designed to read 96-well microplates. All plates adhering to the ANSI/SBS standards should work with the device. 

Can I use U-bottom or V-bottom plates?

Best results are obtained using flat-bottom plates. Our internal tests with U-bottom plates show the same performance with clear samples as compared to flat-bottom plates and can be recommend. If you plan to use V-bottom plates, you need to validate the performance of the reader-plate combination prior usage.

Does the Absorbance 96 Automate need to be maintained or serviced by a technician?

No, the Absorbance 96 Automate is maintenance free.

How can I calibrate the Absorbance 96 Automate?

The Absorbance 96 has many self-checks built in and does not need to be regularly checked for accuracy. However, we are aware that certain laboratory certifications require measurement devices to be regularly calibrated. For this purpose, we recommend the calibration plates from Hellma or our Care Packages, which offer a manufacturer calibration and corresponding certificate. 

Is IQ/OQ/PQ Documentation available for the Absorbance 96 Automate?

Yes, Byonoy can provide the IQ/OQ/PQ documentation upon request. 

Features/Performance

Can I measure fluorescence or luminescence with Absorbance 96 Automate?

No. Absorbance 96 Automate is designed for absorbance (OD) measurements and is not suited to read luminescence or fluorescence. But let’s see what the future brings – if you want to stay in contact and be one of the first to hear about upcoming products and innovations, subscribe to our newsletter.

Does Absorbance 96 Automate offer temperature control?

No.

Is Absorbance 96 Automate verified/validated? Are there studies or comparisons with other readers?

The Absorbance 96, which has a technologically very comparable design to the Absorbance 96 Automate, is being used by laboratory professionals across the world. Comparison data can be provided. If needed, just contact us

Integration

Which systems is Absorbance 96 Automate designed for?

Absorbance 96 Automate is designed to be platform agnostic, meaning it can be integrated into any system that has a microplate gripper. 

Does Absorbance 96 Automate work with any scheduler?

Byonoy is working with well-known scheduler providers to program drivers. If you’re unsure if your scheduler can communicate with our device, please contact us

Does Absorbance 96 Automate have drivers for direct integration into a liquid handler?

Byonoy is working with well-known liquid handler manufacturers to program drivers and enable easy integration. If you want to know if you’re liquid handler is compatible, please contact us.  

How is Absorbance 96 Automate connected to power?

Absorbance 96 Automate uses a USB cable to connect to a computer, which provides both power and data transfer. 

Does Absorbance 96 Automate occupy two deck spaces?

Not quite. Absorbance 96 Automate requires one deck space. In addition, a deck space is needed for the Park Position, where the upper part can be temporarily stored. The Park Position can be freely placed on the deck and is multifunctional since a microplate can additionally be stored on it.

Is Absorbance 96 Automate compatible with Sila2?

Yes. The Absorbance 96 App can be interfaced with via Sila2. For a set of instructions, please contact us

Is it possible to communicate directly with the Absorbance 96 Automate hardware?

Not currently. We enable communication with the device via the Absorbance 96 App, which can be controlled by a scheduler via Sila2 or a driver from the manufacturer of your liquid handler. We plan to offer a hardware API in the future. 

Can I take measurements without paying for external software or drivers?

The Absorbance 96 Automate has an automatic measurement function in the Absorbance 96 app. This function allows readout without a driver. The results are generated and automatically exported to a .csv file, for example.

Applications

Can I use Absorbance 96 Automate for DNA quantification or NGS library prep?

Unfortunately not. Absorbance 96 Automate can’t currently measure UV wavelengths and is not set up for fluorescence readout. 

Can I use a tartrazine dye to perform on-deck volume verification (pipette validation) with Absorbance 96 Automate?

Absolutely! Automate your quality control steps with our on-deck reader module. 

Is Absorbance 96 Automate suitable for protein quantification at 280 nm?

No. Absorbance 96 Automate is designed for colorimetric assays in the visible range, such as ELISA, BCA, Bradford, OD600, or cellular assays such as MTT or XTT. 

Absorbance One

Wavelength/Filters

Can the Absorbance One measure at multiple wavelengths?

No. The Absorbance One is a filter-based, single beam, single-wavelength UV/Vis spectrophotometer. It does not have a monochromator. Each device has a single channel and can offer one specific wavelength, tailored to your application. Examples would be 600 nm for OD600 or 562 nm for BCA. If you need more than one wavelength, our concept of compact size and affordable price makes it possible to buy more than one perfectly tailored instrument for your specific applications.

Can I exchange the filter?

The filter for the Absorbance One is not exchangeable. You must decide on the filter you need before purchasing the instrument. If you need more than one filter, our concept of compact size and affordable price makes it possible to buy multiple instruments for your specific applications. 

Is it possible to customize the wavelength before ordering?

Yes. You are free to choose any wavelength between 280–1000 nm.

Do different wavelengths cost different amounts?

Yes. UV wavelengths, such as 280 or 340 nm, cost slightly more than those in the visible spectrum. For more information get a quote.

Proper Use

Is an installation or setup by a technician necessary?

No. You must simply unbox the device, install the app, and the reader is ready to go within a few minutes.

Can multiple cuvette types be used with the Absorbance One?

Yes. All standard cuvettes can be used with the Absorbance One, including 1cm cuvettes, semi-micro, and micro cuvettes.

Does the orientation of the cuvette in the reader matter?

Yes. Pay attention to the arrow shown on top of the instrument.

Does the Absorbance One need to be maintained or serviced by a technician?

No, the Absorbance One is maintenance free.

Can the Absorbance One be disinfected for sterile usage?

Yes. The Absorbance One can be disinfected using a cloth moistened with 70 % Ethanol solution.

Features/Performance

The instrument performed the measurement immediately upon insertion of the cuvette. Is that normal?

Yes. The Absorbance One features an auto cuvette detection, auto-initialization, and auto-measurement feature. As soon as the device detects a cuvette in the beam path, the measurement takes place in a fraction of a second.

Can I measure fluorescence or luminescence with the Absorbance One?

No. The Absorbance One is designed for absorbance (OD) measurements and is not suited to read luminescence or fluorescence. But let’s see what the future brings – if you want to stay in contact and be one of the first to hear about upcoming products and innovations, subscribe to our newsletter.

Does the Absorbance One have temperature control?

No.

Can I use the Absorbance One in an anaerobic chamber or glove box?

Yes, this is possible and encouraged! Thanks to its small size, the Absorbance One fits into space-restricted enclosures easily.

Is the open slot design susceptible to dust?

Under laboratory condition, only minimal dust exposure is expected to enter the slot, which will be factored out during the initialization procedure. Beyond that, the Absorbance One has an internal dust detection, which will warn you if too much dust is detected, thereby preventing any false results. If needed, simply follow the cleaning procedure detailed in the manual.

Can ambient light disturb the read out?

Byonoy’s patent-pending ambient light canceling technology and advanced data processing enable the cancelation of the ambient light during the readout. That means, that no ambient light can disturb your readout!

Is the Absorbance One verified/validated? Are there studies or comparisons with other readers?

The Absorbance One has been validated by Byonoy internally and has also been rigorously tested by external partners. Comparison data can be provided. If needed, just contact us.

Payment & Shipping

Payment

What payment methods are available?

You can pay by wire transfer (ACH/ABA) or credit card.

Shipping

Does Byonoy ship to my location?

We ship to all locations served by UPS worldwide shipping. We also have an expansive distributor network which offers on-site services in many countries.

How much does shipping cost?

We're happy to offer free door-to-door shipping to your location, wherever that may be.

Service & Repair

Service

Do you offer extended warranty or other “peace of mind” options?

Although our devices are maintenance-free, we do offer additional services to our customers in the form of our Care Packages. For more information, please contact us

How long is the warranty period?

We offer you a 24 months warranty period, starting from the date of delivery.

Repair

If I have an issue with my device, what should I do?

In the unlikely event that you encounter an issue with your device, you can contact our support team at service@byonoy.com or via the Contact Form on our website. We will respond within 48 hours.

Haven’t found the answer you’re looking for?

If our help section did not provide you with a suitable answer, use the contact form to describe your request. We will get back to you soon.

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