Applications Library

Match Your Assay to Your Reader

01

Absorbance

The workhorse of the lab. Compares the light entering the sample to the light exiting the sample. Any light absorbed, scattered, or otherwise attenuated results in an increase in signal (OD).

14assays
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One wavelength per assay
Absorbance 96: four filters, 400–1000 nm
AssayResources
Read wavelength
  1. 412 nm
    412 nm1 paper
  2. 450 nm
    450 nm2 app notes3 validations25 papers
  3. 450 nm
    450 nm4 papers
  4. 450 nm
    450 nmApp note2 papers
  5. 490 nm
    490 nm1 paper
  6. 490 nm
    490 nmValidation2 papers
  7. 562 nm
    562 nmApp note2 papers
  8. 570 nm
    570 nm25 papers
  9. 595 nm
    595 nmApp note1 paper
  10. 595 nm
    595 nm1 paper
  11. 600 nm
    600 nm2 app notesValidation14 papers
  12. 620 nm
    620 nm1 paper
  13. 630 nm
    630 nmApp note
  14. 750 nm
    750 nmApp note

Compatible Absorbance Readers

Absorbance 96 Absorbance 96 Absorbance One Absorbance One Absorbance 96 Automate Absorbance 96 Automate

02

Luminescence

Light emitted by the sample itself (e.g. due to a chemical or biochemical reaction) is collected by a sensitive detector. Total light within the range of the detector is collected and reported as Relative Light Units (RLU).

5assays
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Window 350–650 nm
Most sensitive near 460 nm
Infrared filter from 650 nm
AssayResources
Emission peak
  1. Emission peak 460 nm
    Validation1 paper
  2. Emission peak 460 nm
    1 paper
  3. Emission peak 560 nm
    Validation
  4. Emission peak 560 nm
    Validation
  5. Emission peak 560 nm
    App note1 paper

Compatible Luminescence Readers

Luminescence 96 Luminescence 96 Luminescence 96 Automate Luminescence 96 Automate

03

Fluorescence

One light in, a different light out. A dye works when it is excited inside the blue band and glows inside the green one; each line runs from a dye's excitation peak to its emission peak.

46applications
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Excitation 468–489 nm
Emission 512–584 nm
LED, λdom 482.5 nm
ApplicationFlag
Excitation peak Emission peak
  1. Cyto-ID Green Ex 463 · Em 534 nm
    Good
  2. 2-NBDG Ex 465 · Em 540 nm
    Good
  3. CBQCA Ex 465 · Em 550 nm
    Good
  4. AccuGreen dsDNA Ex 468 · Em 507 nm · Emission peak below 512 nm cut-on
    Validate first
  5. Qubit Protein / NanoOrange Ex 470 · Em 570 nm
    Good
  6. SYPRO Orange (endpoint) Ex 470 · Em 570 nm
    Good
  7. CyQUANT (low cell no.) Ex 480 · Em 520 nm · Lowest cell numbers not reachable
    Acceptable
  8. SYTO 9 Ex 483 · Em 503 nm · Emission peak below 512 nm cut-on
    Validate first
  9. TurboGFP / ZsGreen Ex 483 · Em 503 nm · Emission peak below 512 nm cut-on
    Validate first
  10. DiOC6(3) Ex 484 · Em 501 nm · Emission peak below 512 nm cut-on
    Validate first
  11. CellROX Green Ex 485 · Em 520 nm
    Good
  12. Nile Red (neutral lipids) Ex 485 · Em 555 nm
    Good
  13. EGFP (bacteria/yeast) Ex 488 · Em 507 nm
    Good
  14. EGFP (mammalian) Ex 488 · Em 507 nm · Near LOD vs. autofluorescence
    Acceptable
  15. FDP / FDG Ex 490 · Em 514 nm
    Good
  16. MitoTracker Green Ex 490 · Em 516 nm
    Good
  17. FITC-dextran Ex 490 · Em 520 nm
    Good
  18. SensoLyte 520 (5-FAM FRET) Ex 490 · Em 520 nm
    Good
  19. CFSE / CellTrace Ex 492 · Em 517 nm
    Good
  20. QuantiFluor RNA Ex 492 · Em 540 nm
    Good
  21. BODIPY 493/503 Ex 493 · Em 503 nm · ~35% of emission captured
    Validate first
  22. Fluo-4 / Cal-520 (no injector) Ex 494 · Em 516 nm · No fast flux with compound addition
    Good
  23. Calcein AM Ex 494 · Em 517 nm
    Good
  24. Annexin V-FITC Ex 494 · Em 518 nm · Surface label, weak signal
    Acceptable
  25. Fluorescein / FITC Ex 494 · Em 518 nm
    Good
  26. DAF-FM (NO) Ex 495 · Em 515 nm
    Good
  27. LAMP endpoint (calcein) Ex 495 · Em 515 nm
    Good
  28. Alexa Fluor 488 Ex 495 · Em 519 nm
    Good
  29. CRISPR Cas12/13 FAM reporter Ex 495 · Em 520 nm
    Good
  30. H2DCFDA (ROS) Ex 495 · Em 529 nm
    Good
  31. Rhodamine 110 Ex 497 · Em 520 nm
    Good
  32. SYBR Green I / EvaGreen Ex 498 · Em 522 nm
    Good
  33. Caspase-3/7 Rh110 (Apo-ONE) Ex 499 · Em 521 nm
    Good
  34. CellTox Green Ex 500 · Em 525 nm
    Good
  35. OliGreen standard range Ex 500 · Em 525 nm · Low range not reachable
    Acceptable
  36. RiboGreen high range Ex 500 · Em 525 nm
    Good
  37. RiboGreen low range Ex 500 · Em 525 nm · Est. floor ~10 ng/mL
    Acceptable
  38. PicoGreen low range Ex 502 · Em 523 nm · Kit floor 25 pg/mL; est. ~1 ng/mL
    Acceptable
  39. Qubit 1X dsDNA HS (low end) Ex 502 · Em 523 nm · Bottom of kit range below LOD
    Acceptable
  40. Qubit / Quant-iT dsDNA BR Ex 502 · Em 523 nm
    Good
  41. CellEvent Caspase-3/7 Ex 502 · Em 530 nm
    Good
  42. BODIPY FL substrates Ex 503 · Em 512 nm · ~40–55% of emission captured
    Validate first
  43. SYTOX Green Ex 504 · Em 523 nm
    Good
  44. pHrodo Green Ex 505 · Em 525 nm
    Good
  45. mNeonGreen (microbial) Ex 506 · Em 517 nm · Reduced excitation; bright dye compensates
    Good
  46. EYFP / Venus / Citrine Ex 515 · Em 528 nm · Weak excitation with V1 LED
    Validate first

Compatible Fluorescence Readers

Fluorescence 96 Fluorescence 96

Research from labs that measure with Byonoy readers.

All 214 publications
214Papers Journal papers, preprints and theses from labs using Byonoy readers.