Validation by: Dr. Mariano Viapiano and team (viapianolab.org)
Associate Professor of Neuroscience and Physiology, Neurosurgery, and Cell and Developmental Biology at the State University of New York (SUNY) - Upstate Medical University
Luminescence 96 was evaluated for its performance in cell viability and luciferase-based assays using two commercially available assay systems from Promega:
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CellTiter-Glo®: ATP-based cell viability assays performed on glioblastoma and immune cells.
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Bright-Glo®: Firefly luciferase-based assays used to assess luciferase activity as an indicator of tumor cell viability.
Different cell lines were seeded in standard 96-well plates, and both assays were performed according to their respective protocols. The same plates were measured using the Byonoy Luminescence 96 and an Agilent BioTek Synergy H1 luminometer for direct performance comparison.
Measurements were conducted at room temperature using both white-walled and transparent microplates.
Results: The Luminescence 96 consistently delivered reliable luminescence measurements across both assay types and plate formats, with no detectable signal cross-talk between wells.
These results demonstrate the suitability of Luminescence 96 for ATP-based cell viability measurements and firefly luciferase reporter assays, supporting its use in routine cell-based research workflows.
Photo: Experimental setup used by Dr. Mariano Viapiano and his research team
